Functional Analysis of PsAvr3c Effector Family From Phytophthora Provides Probes to Dissect SKRP Mediated Plant Susceptibility.
Identifieur interne : 000771 ( Main/Exploration ); précédent : 000770; suivant : 000772Functional Analysis of PsAvr3c Effector Family From Phytophthora Provides Probes to Dissect SKRP Mediated Plant Susceptibility.
Auteurs : Ying Zhang [République populaire de Chine] ; Jie Huang [République populaire de Chine] ; Sylvans O. Ochola [République populaire de Chine] ; Suomeng Dong [République populaire de Chine]Source :
- Frontiers in plant science [ 1664-462X ] ; 2018.
Abstract
PsAvr3c is an effector identified from oomycete plant pathogen Phytophthora sojae that causes soybean root and stem rot disease. Earlier studies have demonstrated that PsAvr3c binds to a novel soybean spliceosomal complex protein, GmSKRP, to reprogram the splicing of hundreds of pre-mRNAs and consequently subvert host immunity. PsAvr3c family genes are present in some other Phytophthora species, but their function remains unknown. Here, we characterized the functions of PsAvh27b (PsAvr3c paralog from P. sojae), ProbiAvh89 and PparvAvh214 (orthologs from P. cinnamomi var. robiniae and Phytophthora parvispora, respectively). The study reveals that both PsAvh27b and ProbiAvh89 interact with GmSKRPs in vitro, and stabilize GmSKRP1 in vivo. However, PparvAvh214 cannot interact with GmSKRPs proteins. The qRT-PCR result illustrates that the alternative splicing of pre-mRNAs of several soybean defense-related genes are altered in PsAvh27b and ProbiAvh89 when over-expressed on soybean hairy roots. Moreover, PsAvr3c family members display differences in promoting Phytophthora infection in a SKRP-dependent manner. Overall, this study highlights that the effector-mediated host pre-mRNA alternative splicing occurs in other pathosystems, thus providing new probes to further dissect SKRP-mediated plant susceptibility.
DOI: 10.3389/fpls.2018.01105
PubMed: 30090111
PubMed Central: PMC6069499
Affiliations:
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Ochola</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation><nlm:affiliation>Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing, Chxsina.</nlm:affiliation><wicri:noCountry code="subField">Chxsina</wicri:noCountry></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">PsAvr3c is an effector identified from oomycete plant pathogen <i>Phytophthora sojae</i> that causes soybean root and stem rot disease. Earlier studies have demonstrated that PsAvr3c binds to a novel soybean spliceosomal complex protein, GmSKRP, to reprogram the splicing of hundreds of pre-mRNAs and consequently subvert host immunity. PsAvr3c family genes are present in some other <i>Phytophthora</i> species, but their function remains unknown. Here, we characterized the functions of <i>PsAvh27b</i> (<i>PsAvr3c</i> paralog from <i>P. sojae</i>), <i>ProbiAvh89</i> and <i>PparvAvh214</i> (orthologs from <i>P. cinnamomi</i> var. <i>robiniae</i> and <i>Phytophthora parvispora</i>, respectively). The study reveals that both PsAvh27b and ProbiAvh89 interact with GmSKRPs <i>in vitro</i>, and stabilize GmSKRP1 <i>in vivo</i>. However, PparvAvh214 cannot interact with GmSKRPs proteins. The qRT-PCR result illustrates that the alternative splicing of pre-mRNAs of several soybean defense-related genes are altered in <i>PsAvh27b</i> and <i>ProbiAvh89</i> when over-expressed on soybean hairy roots. Moreover, PsAvr3c family members display differences in promoting <i>Phytophthora</i> infection in a SKRP-dependent manner. Overall, this study highlights that the effector-mediated host pre-mRNA alternative splicing occurs in other pathosystems, thus providing new probes to further dissect SKRP-mediated plant susceptibility.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30090111</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Functional Analysis of PsAvr3c Effector Family From <i>Phytophthora</i> Provides Probes to Dissect SKRP Mediated Plant Susceptibility.</ArticleTitle><Pagination><MedlinePgn>1105</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2018.01105</ELocationID><Abstract><AbstractText>PsAvr3c is an effector identified from oomycete plant pathogen <i>Phytophthora sojae</i> that causes soybean root and stem rot disease. Earlier studies have demonstrated that PsAvr3c binds to a novel soybean spliceosomal complex protein, GmSKRP, to reprogram the splicing of hundreds of pre-mRNAs and consequently subvert host immunity. PsAvr3c family genes are present in some other <i>Phytophthora</i> species, but their function remains unknown. Here, we characterized the functions of <i>PsAvh27b</i> (<i>PsAvr3c</i> paralog from <i>P. sojae</i>), <i>ProbiAvh89</i> and <i>PparvAvh214</i> (orthologs from <i>P. cinnamomi</i> var. <i>robiniae</i> and <i>Phytophthora parvispora</i>, respectively). The study reveals that both PsAvh27b and ProbiAvh89 interact with GmSKRPs <i>in vitro</i>, and stabilize GmSKRP1 <i>in vivo</i>. However, PparvAvh214 cannot interact with GmSKRPs proteins. The qRT-PCR result illustrates that the alternative splicing of pre-mRNAs of several soybean defense-related genes are altered in <i>PsAvh27b</i> and <i>ProbiAvh89</i> when over-expressed on soybean hairy roots. Moreover, PsAvr3c family members display differences in promoting <i>Phytophthora</i> infection in a SKRP-dependent manner. Overall, this study highlights that the effector-mediated host pre-mRNA alternative splicing occurs in other pathosystems, thus providing new probes to further dissect SKRP-mediated plant susceptibility.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Jie</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ochola</LastName><ForeName>Sylvans O</ForeName><Initials>SO</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Suomeng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing, Chxsina.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>07</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora</Keyword><Keyword MajorTopicYN="N">PsAvr3c</Keyword><Keyword 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IdType="doi">10.3389/fpls.2018.01105</ArticleId><ArticleId IdType="pmc">PMC6069499</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>PLoS Pathog. 2006 Oct;2(10):e104</Citation><ArticleIdList><ArticleId IdType="pubmed">17040127</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2014 Mar;26(3):1345-59</Citation><ArticleIdList><ArticleId IdType="pubmed">24632534</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2014 Jun 10;15(6):10424-45</Citation><ArticleIdList><ArticleId IdType="pubmed">24918296</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2016 Jan 29;7:10311</Citation><ArticleIdList><ArticleId IdType="pubmed">26822079</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2012 Apr;102(4):348-64</Citation><ArticleIdList><ArticleId IdType="pubmed">22185336</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2015 Jul;105(7):966-81</Citation><ArticleIdList><ArticleId IdType="pubmed">25760519</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2012 May;13(4):329-37</Citation><ArticleIdList><ArticleId IdType="pubmed">22013895</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Genet. 2001 Nov;17(11):611-4</Citation><ArticleIdList><ArticleId IdType="pubmed">11672843</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2007 Jan;8(1):1-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20507474</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2015 May;16(4):413-34</Citation><ArticleIdList><ArticleId IdType="pubmed">25178392</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2014 Dec;27(12):1379-89</Citation><ArticleIdList><ArticleId IdType="pubmed">25387135</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2017 Dec 12;8(1):2051</Citation><ArticleIdList><ArticleId IdType="pubmed">29233978</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2011 Oct;12(8):715-30</Citation><ArticleIdList><ArticleId IdType="pubmed">21726373</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2007;2(4):948-52</Citation><ArticleIdList><ArticleId IdType="pubmed">17446894</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2012 Dec;72(6):882-93</Citation><ArticleIdList><ArticleId IdType="pubmed">22709376</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2017 Apr 3;27(7):981-991</Citation><ArticleIdList><ArticleId IdType="pubmed">28318979</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2009;4(5):e5556</Citation><ArticleIdList><ArticleId IdType="pubmed">19440541</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Microbiol. 2007 Aug;10(4):332-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17707688</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Jul 29;8(7):e70036</Citation><ArticleIdList><ArticleId IdType="pubmed">23922898</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name></noRegion><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name><name sortKey="Huang, Jie" sort="Huang, Jie" uniqKey="Huang J" first="Jie" last="Huang">Jie Huang</name><name sortKey="Ochola, Sylvans O" sort="Ochola, Sylvans O" uniqKey="Ochola S" first="Sylvans O" last="Ochola">Sylvans O. 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